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Missio,Robson Fernando; Caixeta,Eveline Teixeira; Zambolim,Eunize Maciel; Pena,Guilherme Ferreira; Ribeiro,Ana Paula; Zambolim,Laércio; Pereira,Antônio Alves; Sakiyama,Ney Sussumu. |
EST-SSR markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. Seventeen markers, developed from ESTs (Expressed Sequence Tags) from the Brazilian Coffee Genome Project, were used. All markers showed polymorphism among the genotypes assessed. The average number of allele per primer was 5.1. The highest polymorphisms were found within C. canephora (88.2%) and rust-resistant varieties (35.3%). About 29.4% of the markers differentiated C. arabica from Híbrido de Timor; it was also possible to identify those closest and farthest from C. arabica . The analysis of population-grouped genotypes revealed a 64.0% genetic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA markers; Coffea sp.; UPGMA; Amova; Microsatellite markers. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052009000300003 |
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Partelli,Fábio Luiz; Vieira,Henrique Duarte; Rodrigues,Ana Paula Dias; Pais,Isabel; Campostrini,Eliemar; Chaves,Maria Manuela Coelho Cabral; Ramalho,José Cochicho. |
The present research aimed to characterize some biochemical responses of Coffea canephora (clones 02 and 153) and C. arabica (Catucaí IPR 102) genotypes subjected to low positive temperatures, helping to elucidate the mechanisms involved in cold tolerance. For that, one year old plants were subjected successively to 1) a temperature decrease (0.5°C a day) from 25/20°C to 13/8°C (acclimation period), 2) a three day chilling cycle (3x13/4°C) and to 3) a recovery period of 14 days (25/20°C). In Catucaí (less cold sensitive when compared to clone 02) there was an increased activity in the respiratory enzymes malate dehydrogenase and pyruvate kinase. Furthermore, Catucaí showed significant increases along the cold imposition and the higher absolute values after... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffee tree; Coffea sp.; Sucrose; Enzymes; Cold stress. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400006 |
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